TANDEM MASS-SPECTROMETRY OF CARBONIC-ANHYDRASE (29-KDA)

被引：52

作者：

OCONNOR, PB ^{[1
]}

SPEIR, JP ^{[1
]}

SENKO, MW ^{[1
]}

LITTLE, DP ^{[1
]}

MCLAFFERTY, FW ^{[1
]}

机构：

[1] CORNELL UNIV,BAKER LAB,DEPT CHEM,ITHACA,NY 14853

来源：

JOURNAL OF MASS SPECTROMETRY | 1995年 / 30卷 / 01期

关键词：

D O I：

10.1002/jms.1190300114

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using electrospray ionization with a Fourier-transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings, Such sensitive and specific direct characterization of proteins appears to be complementary to conventional techniques including LC/MS/MS sequencing of oligopeptides from protein degradation.

引用

页码：88 / 93

页数：6

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