THE SULFATED CARBOHYDRATE-PROTEIN LINKAGE REGION ISOLATED FROM CHONDROITIN 4-SULFATE CHAINS OF INTER-ALPHA-TRYPSIN INHIBITOR IN HUMAN PLASMA

被引：37

作者：

YAMADA, S

OYAMA, M

KINUGASA, H

NAKAGAWA, T

KAWASAKI, T

NAGASAWA, S

KHOO, KH

MORRIS, HR

DELL, A

SUGAHARA, K

机构：

[1] KOBE PHARMACEUT UNIV, DEPT BIOCHEM, KOBE 658, JAPAN

[2] KYOTO UNIV, FAC PHARMACEUT SCI, DEPT BIOL CHEM, KYOTO 606, JAPAN

[3] HOKKAIDO UNIV, FAC PHARMACEUT SCI, DEPT HYG CHEM, SAPPORO, HOKKAIDO 060, JAPAN

[4] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED, DEPT BIOCHEM, LONDON, ENGLAND

来源：

GLYCOBIOLOGY | 1995年 / 5卷 / 03期

基金：

英国惠康基金;

关键词：

CHONDROITIN SULFATE; FAB-MS; INTER-ALPHA-TRYPSIN INHIBITOR; SULFATED OLIGOSACCHARIDES;

D O I：

10.1093/glycob/5.3.335

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inter-alpha-trypsin inhibitor (ITI) in human plasma has a unique structural architecture composed of three polypeptide chains (H-1, H-2 and L chains), which are linked to each other through a chondroitin 4-sulphate chain. The structure of the carbohydrate-protein linkage region of the chondroitin 4-sulphate chain attached to the L chain was investigated. The peptide-chondroitin sulphate fraction was isolated by anion-exchange chromatography after exhaustive digestion with lysyl endopeptidase and then V8 protease. The chondroitin 4-sulphate chain was released from the peptides by beta-elimination using (NaBH4)-H-3 and then digested with chondroitinase ABC. These treatments resulted in a single H-3-labelled hexasaccharide alditol fraction derived from the linkage region which had been associated with the L chain. Chemical and enzymatic analyses as well as fast-atom bombardment-mass spectrometry (FAB-MS) analysis revealed that the H-3-labelled hexasaccharide alditol had the following structure: Delta HexA-alpha 1-3GalNAc(4-sulphate) beta 1-4GlcA beta 1-3Gal(4-sulphate)beta 1-3Gal beta 1-4Xyl-ol (where Delta HexA is 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and Xyl-ol is xylitol). The structure contained the novel 4-sulphated Gal residue, which was previously demonstrated in a linkage hexasaccharide isolated from chondroitin 4-sulphate of rat chondrosarcoma (Sugahara et al., J, Biol. Chem., 263, 10168-10174, 1988) and of whale cartilage (Sugahara et al., Eur. J. Biochem., 202, 805-811, 1991). The above disulphated hexasaccharide alditol was the only component detected in the linkage region fraction of the chondroitin 4-sulphate chain of ITI, which implies some biological significance of this novel structure.

引用

页码：335 / 341

页数：7

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