IMMOBILIZED NUCLEOTIDES AND COENZYMES FOR AFFINITY CHROMATOGRAPHY

General methods for the preparation of coenzyme and nucleotide analogues substituted with spacer molecules at various positions on the purine, phosphate and ribose moieties and suitable for attachment to insoluble support matrices are given. The relative efficiencies of the resulting differently immobilised nucleotides are compared both in regard to the position of substitution and in terms of the relative merits of whole coenzyme immobilisation versus the “AMP-half”. Both the biospecificity and and the problem of non-specific adsorption to nucleotide adsorbents is discussed. Finally, examples are given to illustrate the range of potential applications of immobilised coenzymes. © 1979, Walter de Gruyter. All rights reserved.