CRYOSECTIONING OF UNDECALCIFIED TISSUES FOR IMMUNOFLUORESCENCE

被引：39

作者：

RIJNTJES, NVM

VANDEPUTTE, LBA

VANDERPOL, M

GUELEN, PJM

机构：

[1] CATHOLIC UNIV NIJMEGEN,ST RADBOUD HOSP,DEPT RHEUMATOL,NIJMEGEN,NETHERLANDS

[2] CATHOLIC UNIV NIJMEGEN,ST RADBOUD HOSP,DEPT CLIN PHARM,NIJMEGEN,NETHERLANDS

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 30卷 / 03期

关键词：

D O I：

10.1016/0022-1759(79)90100-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present report describes a procedure for preparing 4-6 μm cryostat sections of undecalcified fresh frozen tissue which contain hard tissue, for immunofluorescence. The apparatus used is a cryomicrotone originally designed for cutting sections for whole body autoradiography. To obtain cryostat sections suitable for tissue immunofluorescence the standard procedure was modified with respect to the hardness and edges of the microtome knife, the temperature of the cryostat and the carbomethyl cellulose concentration of the embedding material. © 1979.

引用

页码：263 / 268

页数：6

共 7 条

[1] ANTIGEN-INDUCED ARTHRITIS IN MICE .1. INDUCTION OF ARTHRITIS IN VARIOUS STRAINS OF MICE [J].