DETERMINATION OF PHOLCODINE AND ITS METABOLITES IN URINE BY CAPILLARY GAS-CHROMATOGRAPHY

被引：22

作者：

JOHANSEN, M ^{[1
]}

RASMUSSEN, KE ^{[1
]}

CHRISTOPHERSEN, AS ^{[1
]}

机构：

[1] NATL INST FORENS TOXICOL,N-0320 OSLO 3,NORWAY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 532卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)83778-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and selective method for the determination of pholcodine and its metabolites in urine using capillary gas chromatography with nitrogen detection is described. The procedure includes enzymatic hydrolysis of urine by β-glucuronidase and sample pretreatment on C2 solid-phase extraction columns. Validation of the method showed good sensitivity, precision and reproducibility. The method was useful for the study of pholcodine metabolism in man. Pholcodine was found to conjungate with glucuronic acid. Morphine was identified as a metabolite and another unidentified metabolite was also detected. © 1990.

引用

页码：277 / 284

页数：8

共 7 条

[1]

Eddy, Friebel, Hahn, Halbach, Bull. WHO, 40, (1969)

[2]

Svenneby, Wedege, Karlsen, Forensic Sci. Int., 21, (1983)

[3]

Findlay, Fowle, Butz, Jones, Weatherley, Welch, Posner, Br. J. Clin. Pharmacol., 22, (1986)

[4]

Chen, Siebert, Somogyi, Bochner, Determination of pholcodine in biological fluids by high-performance liquid chromatography with fluorescence detection, Journal of Chromatography B: Biomedical Sciences and Applications, 424, (1988)

[5]

Combie, Blake, Nugent, Tobin, Clin. Chem., 28, (1982)

[6]

Chen, Reynolds, Bochner, Somogyi, J. Chromatogr., 493, (1989)

[7]

Chen, Bochner, Somogyi, Br. J. Clin. Pharmacol., 26, (1988)

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