CHARACTERIZATION OF THE SYRIAN-HAMSTER C-HA-RAS GENE AND INTRON-D-EXON TRANSCRIPT

被引：11

作者：

EBERT, R

WISEMAN, RW

BARRETT, JC

REISS, E

ROLLICH, G

SCHIFFMANN, D

机构：

[1] NIEHS,MOLEC CARCINOGENESIS LAB,MD D2-04,POB 12233,RES TRIANGLE PK,NC 27709

[2] UNIV WURZBURG,INST PHARMACOL & TOXICOL,W-8700 WURZBURG,GERMANY

来源：

MOLECULAR CARCINOGENESIS | 1992年 / 5卷 / 04期

关键词：

CHEMICAL CARCINOGENESIS; HA-RAS; NEGATIVE CONTROL OF GENE EXPRESSION; PROTOONCOGENE; SYRIAN HAMSTER;

D O I：

10.1002/mc.2940050404

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding sequences as well as 5'- and 3'-flanking sequences of the Syrian hamster c-Ha-ras gene were deduced from cDNA clones derived from embryo fibroblast cell lines. Sequences of introns B, C, and D were obtained from genomic DNA after amplification by the polymerase chain reaction. Sequence comparisons with rat, mouse, and human c-Ha-ras genes revealed a high degree of homology. One of 12 cDNA clones contained intron-D-exon (IDX) sequences due to alternative splicing that would encode a p19 Ha-ras gene product. Conservation between species suggests a functional role for the IDX, possibly as a negative control of p21 Ha-ras expression.

引用

页码：254 / 258

页数：5

共 48 条

[1] OVUM AGING AND PH IMBALANCE AS A CAUSE OF CHROMOSOMAL-ANOMALIES IN HAMSTER [J].