AGGREGATION OF BACTERIOCHLOROPHYLL-C HOMOLOGS TO DIMERS, TETRAMERS, AND POLYMERS IN WATER-SATURATED CARBON-TETRACHLORIDE

Three homologs of BChl c, 2-(R)-(1-hydroxyethyl)-4-n-propyl-5-ethyl-farnesyl BChl c (PEF-BChl c), 2-(R)-(1-hydroxyethyl)-4-ethyl-5-ethyl-farnesyl BChl c (EEF-BChl c), and 2-(S)-(1-hydroxyethyl)-4-isobutyl-5-methyl/ ethyl-farnesyl BChl c (iBM/EF-BChl c), formed aggregates in water-saturated carbon tetrachloride (H2O-satd CCl4). The water content was about 100 times higher than that of the dried CCl4 previously used. Absorption spectra were recorded for 8 concentrations for the three homologs of BChl c and were deconvoluted in terms of standard spectra of monomer, dimer, tetramer and polymer (747-nm aggregate, Olson and Pedersen (1990) Photosynth Res 25: 25). PEF- and EEF-BChl c formed dimers (680 nm maximum) and tetramers (705-710 nm maximum), but iBM/EF-BChl c formed polymers. Inhibition of dimer formation by water facilitated the study of the initial stages of the polymerization of BChl c. When the logarithm of polymer concentration was plotted versus the logarithm of the monomer concentration for iBM/EF-BChl c, the initial slope was 30 +/- 10 and indicated the cooperation of 20-40 BChl c molecules to form a polymer from a seed. Circular dichroism spectra of the polymers with positive and negative bands at 743 and 760 nm, respectively, were similar to those for chlorosomes (Brune et al. (1990) Photosynth Res 24: 253).