SELECTIVE REMOVAL OF ALPHA-D-GALACTOSE SIDE-CHAINS FROM RHIZOBIUM CAPSULAR POLYSACCHARIDE BY GUAR ALPHA-D-GALACTOSIDASE - EFFECT ON CONFORMATIONAL STABILITY AND GELATION

被引:21
作者
GIDLEY, MJ
EGGLESTON, G
MORRIS, ER
机构
[1] SILSOE COLL,CRANFIELD INST TECHNOL,SILSOE MK45 4DT,BEDS,ENGLAND
[2] UNILEVER RES LTD,COLWORTH LAB,SHARNBROOK MK44 1LQ,BEDS,ENGLAND
关键词
D O I
10.1016/0008-6215(92)84018-N
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
alpha-D-Galactosidase, isolated from germinating seeds of guar, removed up to 48% of the (1 --> 2)-linked alpha-D-galactose side chains of Rhizobium capsular polysaccharide (CPS), with no loss of the disaccharide side chains (1 --> 6)-linked to the same backbone residues in the hexasaccharide repeating units. Results from differential scanning calorimetry (DSC) and measurements of gel rigidity (G') indicate that removal of alpha-D-galactose side chains facilitates adoption of the "pseudo double-helical" structure proposed from X-ray fibre diffraction analysis (increase in T(m) and reduction in DSC peak width for conformational ordering on cooling), but progressively eliminates the helix-helix aggregation necessary for gel formation (reduction in thermal hysteresis and in G').
引用
收藏
页码:185 / 196
页数:12
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