ULTRASTRUCTURAL CHARACTERIZATION AND 3-DIMENSIONAL RECONSTRUCTION OF ISOLATED MAIZE (ZEA-MAYS L) EGG CELL PROTOPLASTS

被引:43
作者
FAURE, JE
MOGENSEN, HL
KRANZ, E
DIGONNET, C
DUMAS, C
机构
[1] UNIV LYON 1, CNRS, EP 20, INRA, LA 879, BAT 741, F-69622 VILLEURBANNE, FRANCE
[2] NO ARIZONA UNIV, DEPT BIOL SCI, FLAGSTAFF, AZ 86011 USA
[3] UNIV HAMBURG, INST ALLGEMEINE BOT, W-2000 HAMBURG 13, GERMANY
关键词
EGG CELL; FEMALE GAMETE; ORGANELLE DISTRIBUTION; 3-DIMENSIONAL RECONSTRUCTION; TRANSMISSION ELECTRON MICROSCOPY; ZEA-MAYS L;
D O I
10.1007/BF01403723
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Isolated egg cell protoplasts of Zea mays L., inbred line A 188, have been studied at the transmission electron microscope level. Their preparation for electron microscopy has been performed by embedding in ultra-low gelling agarose as a preliminary step. Five isolated egg cell protoplasts were serially ultrathin sectioned and studied in detail. One of these protoplasts was reconstructed in three dimensions to provide additional information on its structure. After enzymatic digestion and microdissection, isolated egg cells are true, highly vacuolized protoplasts. The structure of their organelles agrees with in situ observations, indicating an ultrastructural intactness after isolation: the mitochondria are polymorphic, form reticulate networks, and have well developed cristae; the plastids contain starch grains; and the spherical nucleus is euchromatic. As in situ, the organelles of the isolated egg cell protoplasts are aggregated near the nucleus. The complete picture provided by this work should serve as a comparative base for studies on in vitro fertilization products.
引用
收藏
页码:97 / 103
页数:7
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