UP-REGULATION OF NITRIC-OXIDE SYNTHASE BY ESTRADIOL IN HUMAN AORTIC ENDOTHELIAL-CELLS

被引：336

作者：

HISHIKAWA, K

NAKAKI, T

MARUMO, T

SUZUKI, H

KATO, R

SARUTA, T

机构：

[1] KEIO UNIV,SCH MED,DEPT PHARMACOL,SHINJUKU KU,TOKYO 160,JAPAN

[2] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN

来源：

FEBS LETTERS | 1995年 / 360卷 / 03期

关键词：

NITRIC OXIDE; HUMAN ENDOTHELIAL CELL; ESTROGEN; NO SYNTHASE; TESTOSTERONE; ATHEROSCLEROSIS;

D O I：

10.1016/0014-5793(95)00124-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment,vas accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.

引用

页码：291 / 293

页数：3

共 19 条

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