CRYOPRESERVATION OF ZYGOTIC EMBRYOS AND KERNELS OF OIL PALM (ELAEIS-GUINEENSIS JACQ)

被引:18
作者
ENGELMANN, F [1 ]
CHABRILLANGE, N [1 ]
DUSSERT, S [1 ]
DUVAL, Y [1 ]
机构
[1] ORSTOM, F-34032 MONTPELLIER 01, FRANCE
关键词
CRYOPRESERVATION; DESICCATION; OIL PALM; REHYDRATION; KERNELS; ZYGOTIC EMBRYOS;
D O I
10.1017/S0960258500002658
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In the present study, the efficiency of two cryopreservation strategies for oil palm (Elaeis guineensis Jacq.) was compared. If extracted from rehydrated kernels, 65% of the embryos desiccated to around 0.3 g H2O/g DW developed into plantlets after cryopreservation. In contrast, only 25% of embryos (0.12 g H2O/g DW) extracted from cryopreserved dry kernels developed into plantlets. However, this value was increased to 63% if kernels were partially rehydrated before freezing until the water content of embryos reached 0.3 g H2O/g DW. This study emphasizes the importance of partial rehydration of oil palm embryos before cryopreservation.
引用
收藏
页码:81 / 86
页数:6
相关论文
共 22 条
[1]  
Abdeinour A., Villalobos V., Engelmann F., Cryopreservation of zygotic embryos of Coffea spp, Cryo Letters, 13, pp. 297-302, (1992)
[2]  
Assy-Bah B., Engelmann F., Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of plantlets, Cryo Letters, 13, pp. 117-126, (1992)
[3]  
Bewley J.D., Black M., Physiology and biochemistry of seeds in relation to germination, Development, germination and growth, 1, (1983)
[4]  
Chin H.F., Krishnappillay B., Alang Z.C., Cryopreservation of Veitchia and Howea palm embryos: nondevelopment of the haustorium, Cryo Letters, 9, pp. 372-379, (1988)
[5]  
Crowe J.H., Crowe L.M., Stabilisation of membranes in anhydrobiotic organisms, pp. 188-209, (1986)
[6]  
Dumet D., Engelmann F., Chabrillange N., Duval Y., Cryopreservation of standard oil palm (Elaeis guineensis Jacq.), Plant Cell Reports, 12, pp. 352-355, (1993)
[7]  
Dumet D., Engelmann F., Chabrillange N., Duval Y., Development of cryopreservation for oil palm somatic embryos using an improved process, Oléagineux, 48, pp. 273-278, (1993)
[8]  
Ellis R.H., Hong T.D., Roberts E.H., Soetisna U., Seed storage behaviour in Elaeis guineensis, Seed Science Research, 1, pp. 99-104, (1991)
[9]  
Engelmann F., Cryopreservation of embryos, pp. 281-290, (1992)
[10]  
Grout B.W.W., Wescott R.J., Pritchard H.W., Orthodox behaviour of oil palm seeds and cryopreservation of the excised embryo for genetic conservation, Annals of Botany, 52, pp. 381-384, (1983)