One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin

被引：75

作者：

Crowe, J ^{[1
]}

Masone, BS ^{[1
]}

Ribbe, J ^{[1
]}

机构：

[1] QIAGEN INC,CHATSWORTH,CA 91311

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 03期

关键词：

protein expression; metal chelate chromatography; purification of native proteins; purification of denatured proteins; protein minipreps;

D O I：

10.1007/BF02779018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.

引用

收藏

页码：247 / 258

页数：12

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