SIMPLE ISOLATION OF DNA HYDROPHOBICALLY COMPLEXED WITH PRESUMED GENE REGULATORY PROTEINS (M3)

Chromatin from chicken reticulocytes and mouse Ehrlich ascites tumor cells has been extracted with 2 M NaCl, leaving a portion of the DNA still complexed with a fraction of nonhistones (designated M3, since it can be dissociated from DNA in solutions of 3 M NaCl containing 5 M urea). The DNA complexed with M3, separated from the bulk DNA by centrifugation, was found to contain sequences poorly represented in bulk DNA. Specifically we found that DNA-M3 complexes isolated from chicken reticulocyte chromatin were enriched in globin gene sequences by 20-fold relative to un-fractionated DNA and by over 1000-fold relative to DNA rendered free of protein following the extraction of chromatin with 2 M NaCl. We have therefore isolated DNA fractions complexed with M3 which are enriched in specific sequences as may be determined by M3. © 1979, American Chemical Society. All rights reserved.