A LIPID LIPASE AGGREGATE WITH ETHER LINKAGE AS A NEW TYPE OF IMMOBILIZED ENZYME FOR ENANTIOSELECTIVE HYDROLYSIS IN ORGANIC-SOLVENTS

被引：14

作者：

AKITA, H ^{[1
]}

UMEZAWA, I ^{[1
]}

MATSUKURA, H ^{[1
]}

OISHI, T ^{[1
]}

机构：

[1] INST PHYS & CHEM RES, WAKO, SAITAMA 35101, JAPAN

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 1992年 / 40卷 / 02期

关键词：

ENANTIOSELECTIVE HYDROLYSIS; WATER-INSOLUBLE SUBSTRATE; ALPHA-ACYLOXYESTER; LIPID LIPASE AGGREGATE; DILTIAZEM HYDROCHLORIDE;

D O I：

10.1248/cpb.40.318

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

For the purpose of carrying out smoothly enzymatic reaction of water-insoluble substrates in organic solvents, a new type of immobilized enzyme, a lipid-lipase aggregate, was developed. In order to prepare various kinds of lipid-lipase aggregates, 27 kinds of dialkyl ether-type phospholipid analogues were newly synthesized and used for the preparation of aggregates with lipase. Thus obtained lipid-lipase aggregates were found to catalyze the enantioselective hydrolysis of the (+/-)-alpha-acyloxy ester 2 much more efficiently than lipase immobilized with synthetic prepolymer (ENTP-4000) in water-saturated isopropyl ether. Namely, the reaction time became much shorter (2 to 3 d for completion as compared with 21 d) and the chemical and optical yields of the reaction products were found to be high.

引用

页码：318 / 324

页数：7

共 13 条

[1] SYNTHESES OF H-2-LABELED AND C-13-LABELED 1,2-DI-O-HEXADECYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINES AND 1,2-DI-O-HEXADECYL-SN-GLYCERO-3-PHOSPHOCHOLINES [J].