A SMALL CHLOROPLAST RNA MAY BE REQUIRED FOR TRANSSPLICING IN CHLAMYDOMONAS-REINHARDTII

被引:148
作者
GOLDSCHMIDTCLERMONT, M
CHOQUET, Y
GIRARDBASCOU, J
MICHEL, F
SCHIRMERRAHIRE, M
ROCHAIX, JD
机构
[1] UNIV GENEVA,DEPT MOLEC BIOL,CH-1211 GENEVA 4,SWITZERLAND
[2] INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
[3] CNRS,CTR GENET MOLEC,F-91190 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1016/0092-8674(91)90415-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In C. reinhardtii, the mature psaA mRNA is assembled by a process involving trans-splicing of three separate transcripts encoded at three widely scattered loci of the chloroplast genome. At least one additional chloroplast locus (tscA) is required for trans-splicing of exons 1 and 2. We have mapped this gene by transformation of a deletion mutant with a particle gun. The 0.7 kb region of the chloroplast genome that is sufficient to rescue tscA function has been subjected to insertion mutagenesis, showing that it does not contain significant open reading frames. We suggest from these experiments that the products of the tscA gene may be a small chloroplast RNA that acts in trans in the first trans-splicing reaction of psaA. A model for the mode of action of this RNA is presented, in which the characteristic structure of group II introns is assembled from three separate transcripts.
引用
收藏
页码:135 / 143
页数:9
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