DETERMINATION OF THE 5' EXON STRUCTURE OF THE HUMAN CARDIAC ALPHA-MYOSIN HEAVY-CHAIN GENE

被引：5

作者：

BRAND, NJ

DABHADE, N

YACOUB, M

BARTON, PJR

机构：

[1] Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, SW3 6LY, Dovehouse St

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91707-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have deduced the exon structure of the 5′ untranslated region of the human cardiac α-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5′ non-coding region of the α-myosin heavy chain gene is interupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the α-myosin heavy chain promoter based on comparisons between the rat and human genes. © 1991.

引用

收藏

页码：1255 / 1258

页数：4

相关论文

共 9 条

[1] THE CHICKEN PROGESTERONE-RECEPTOR - SEQUENCE, EXPRESSION AND FUNCTIONAL-ANALYSIS [J].

GRONEMEYER, H ;

TURCOTTE, B ;

QUIRINSTRICKER, C ;

BOCQUEL, MT ;

MEYER, ME ;

KROZOWSKI, Z ;

JELTSCH, JM ;

LEROUGE, T ;

GARNIER, JM ;

CHAMBON, P .

EMBO JOURNAL, 1987, 6 (13) :3985-3994

[2] MOLECULAR-CLONING AND CHARACTERIZATION OF HUMAN CARDIAC ALPHA-FORM AND BETA-FORM MYOSIN HEAVY-CHAIN COMPLEMENTARY-DNA CLONES - REGULATION OF EXPRESSION DURING DEVELOPMENT AND PRESSURE OVERLOAD IN HUMAN ATRIUM [J].

KURABAYASHI, M ;

TSUCHIMOCHI, H ;

KOMURO, I ;

TAKAKU, F ;

YAZAKI, Y .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (02) :524-531

[3] CARDIAC ALPHA-MYOSIN AND BETA-MYOSIN HEAVY-CHAIN GENES ARE ORGANIZED IN TANDEM [J].

MAHDAVI, V ;

CHAMBERS, AP ;

NADALGINARD, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (09) :2626-2630

[4] HUMAN CARDIAC MYOSIN HEAVY-CHAIN GENE MAPPED WITHIN CHROMOSOME REGION 14Q11.2-]Q13 [J].

MATSUOKA, R ;

YOSHIDA, MC ;

KANDA, N ;

KIMURA, M ;

OZASA, H ;

TAKAO, A .

AMERICAN JOURNAL OF MEDICAL GENETICS, 1989, 32 (02) :279-284

[5] HUMAN CARDIAC MYOSIN HEAVY-CHAIN GENES AND THEIR LINKAGE IN THE GENOME [J].

SAEZ, LJ ;

GIANOLA, KM ;

MCNALLY, EM ;

FEGHALI, R ;

EDDY, R ;

SHOWS, TB ;

LEINWAND, LA .

NUCLEIC ACIDS RESEARCH, 1987, 15 (13) :5443-5459

[6] THYROID-HORMONE REGULATES EXPRESSION OF A TRANSFECTED HUMAN ALPHA-MYOSIN HEAVY-CHAIN FUSION GENE IN FETAL-RAT HEART-CELLS [J].

TSIKA, RW ;

BAHL, JJ ;

LEINWAND, LA ;

MORKIN, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :379-383

[7] MOLECULAR-CLONING OF HUMAN CARDIAC TROPONIN-I USING POLYMERASE CHAIN-REACTION [J].

VALLINS, WJ ;

BRAND, NJ ;

DABHADE, N ;

BUTLERBROWNE, G ;

YACOUB, MH ;

BARTON, PJR .

FEBS LETTERS, 1990, 270 (1-2) :57-61

[8] CHARACTERIZATION OF HUMAN CARDIAC MYOSIN HEAVY-CHAIN GENES [J].

YAMAUCHITAKIHARA, K ;

SOLE, MJ ;

LIEW, J ;

ING, D ;

LIEW, CC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3504-3508

[9]

YAMAUCHITAKIHARA K, 1989, P NATL ACAD SCI USA, V86, P7416