PLASMID PLTRPOLY - A VERSATILE HIGH-EFFICIENCY MAMMALIAN EXPRESSION VECTOR

被引：27

作者：

MAKELA, TP

PARTANEN, J

SCHWAB, M

ALITALO, K

机构：

[1] UNIV HELSINKI,DEPT VIROL,CANC BIOL LAB,SF-00290 HELSINKI 29,FINLAND

[2] UNIV HELSINKI,DEPT PATHOL,SF-00290 HELSINKI 29,FINLAND

[3] GERMAN CANC RES CTR,INST EXPTL PATHOL,W-6900 HEIDELBERG 1,GERMANY

来源：

GENE | 1992年 / 118卷 / 02期

关键词：

MOLONEY MURINE LEUKEMIA VIRUS; LONG TERMINAL REPEAT; POLYLINKER;

D O I：

10.1016/0378-1119(92)90203-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pLTRpoly is an expression vector enabling high-level expression of introduced genes in a variety of cell types. A large multiple cloning site (MCS) and the availability of the full-length nucleotide sequence facilitate the generation of constructs using this vector. Here, constructs made with pLTRpoly have been tested in NIH3T3 mouse fibroblasts.

引用

收藏

页码：293 / 294

页数：2

相关论文

共 13 条

[1] L-MYC COOPERATES WITH RAS TO TRANSFORM PRIMARY RAT EMBRYO FIBROBLASTS [J].

BIRRER, MJ ;

SEGAL, S ;

DEGREVE, JS ;

KAYE, F ;

SAUSVILLE, EA ;

MINNA, JD .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2668-2673

[2] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[3] EXON TRAPPING - A GENETIC SCREEN TO IDENTIFY CANDIDATE TRANSCRIBED SEQUENCES IN CLONED MAMMALIAN GENOMIC DNA [J].

DUYK, GM ;

KIM, SW ;

MYERS, RM ;

COX, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) :8995-8999

[4] REGULATION BY EGF IS MAINTAINED IN AN OVEREXPRESSED CHIMERIC EGFR NEU RECEPTOR TYROSINE KINASE [J].

LEHVASLAIHO, H ;

SISTONEN, L ;

DIRENZO, F ;

PARTANEN, J ;

COMOGLIO, P ;

HOLTTA, E ;

ALITALO, K .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1990, 42 (03) :123-133

[5] ALTERNATIVE FORMS OF MAX AS ENHANCERS OR SUPPRESSORS OF MYC-RAS COTRANSFORMATION [J].

MAKELA, TP ;

KOSKINEN, PJ ;

VASTRIK, I ;

ALITALO, K .

SCIENCE, 1992, 256 (5055) :373-377

[6] EFFICIENT EXPRESSION OF RETROVIRAL VECTOR-TRANSDUCED HUMAN LOW-DENSITY LIPOPROTEIN (LDL) RECEPTOR IN LDL RECEPTOR-DEFICIENT RABBIT FIBROBLASTS INVITRO [J].

MIYANOHARA, A ;

SHARKEY, MF ;

WITZTUM, JL ;

STEINBERG, D ;

FRIEDMANN, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6538-6542

[7] A NOVEL ENDOTHELIAL-CELL SURFACE-RECEPTOR TYROSINE KINASE WITH EXTRACELLULAR EPIDERMAL GROWTH-FACTOR HOMOLOGY DOMAINS [J].

PARTANEN, J ;

ARMSTRONG, E ;

MAKELA, TP ;

KORHONEN, J ;

SANDBERG, M ;

RENKONEN, R ;

KNUUTILA, S ;

HUEBNER, K ;

ALITALO, K .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (04) :1698-1707

[8] HUMAN N-MYC GENE CONTRIBUTES TO NEOPLASTIC TRANSFORMATION OF MAMMALIAN-CELLS IN CULTURE [J].

SCHWAB, M ;

VARMUS, HE ;

BISHOP, JM .

NATURE, 1985, 316 (6024) :160-162

[9] NUCLEOTIDE-SEQUENCE OF MOLONEY MURINE LEUKEMIA-VIRUS [J].

SHINNICK, TM ;

LERNER, RA ;

SUTCLIFFE, JG .

NATURE, 1981, 293 (5833) :543-548

[10]

SISTONEN L, 1990, ONCOGENE, V5, P815