ELECTROCHEMICAL PREPARATION OF ACTIVE AVIDIN FILMS FOR ENZYME SENSOR APPLICATIONS

被引：17

作者：

ANZAI, J ^{[1
]}

HOSHI, T ^{[1
]}

OSA, T ^{[1
]}

机构：

[1] TOHOKU UNIV, INST PHARMACEUT, SENDAI, MIYAGI 980, JAPAN

来源：

CHEMISTRY LETTERS | 1993年 / 07期

关键词：

D O I：

10.1246/cl.1993.1231

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A thin film of avidin was prepared on the surface of platinum electrode by applying an alternating potential of a triangular-wave form (38.5 Hz) from -0.5 to +2.1 V vs. Ag/AgCl to the electrode immersed in the avidin solution. The electro-deposited avidin film exhibited a strong ability to bind biotin-labeled glucose oxidase. The optimum conditions for the deposition of avidin film were studied in relation to the application of the film to enzyme sensors.

引用

页码：1231 / 1234

页数：4

共 5 条

[1] ENZYME-MODIFIED LANGMUIR-BLODGETT MEMBRANES IN GLUCOSE ELECTRODES BASED ON AVIDIN-BIOTIN INTERACTION [J].

LEE, SY ;

ANZAI, J ;

OSA, T .

SENSORS AND ACTUATORS B-CHEMICAL, 1993, 12 (02) :153-158

[2] DEHYDROGENASE-MODIFIED CARBON-FIBER MICROELECTRODES FOR THE MEASUREMENT OF NEUROTRANSMITTER DYNAMICS .2. COVALENT MODIFICATION UTILIZING AVIDIN BIOTIN TECHNOLOGY [J].

PANTANO, P ;

KUHR, WG .

ANALYTICAL CHEMISTRY, 1993, 65 (05) :623-630

[3]

PANTARO P, 1991, J AM CHEM SOC, V111, P1832

[4] DESIGN OF A GLUCOSE MINISENSOR BASED ON STREPTAVIDIN GLUCOSE-OXIDASE COMPLEX COUPLING WITH SELF-ASSEMBLED BIOTINYLATED PHOSPHOLIPID MEMBRANE ON SOLID SUPPORT [J].

SNEJDARKOVA, M ;

REHAK, M ;

OTTO, M .

ANALYTICAL CHEMISTRY, 1993, 65 (06) :665-668

[5] THE AVIDIN BIOTIN COMPLEX IN BIOANALYTICAL APPLICATIONS [J].

WILCHEK, M ;

BAYER, EA .

ANALYTICAL BIOCHEMISTRY, 1988, 171 (01) :1-32

← 1 →