PACKING OF A TRIACYLGLUCOLIPID FROM THE MEMBRANE OF ACHOLEPLASMA-LAIDLAWII STRAIN-A AT THE AIR-WATER-INTERFACE

Pressure-area curves were generated at 22-degrees-C and 40-degrees-C for three glucolipids isolated from Acholeplasma laidlawii strain A-EF22. The glucolipids are 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDAG), 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1 --> 2)-O-alpha-D-glucopyrnaosyl]-sn-glycerol (DGlcDAG), and 1,2-diacyl-3-O-[3-O-acyl-(alpha-D-glucopyranosyl)]-sn-glycerol (MAMGlcDAG). The curves for MGlcDAG and DGlcDAG are characteristic for monolayers in a liquid phase at both temperatures. MGlcDAG has a smaller molecular area at all surface pressures compared to DGlcDAG. At 22-degrees-C MAMGlcDAG shows a phase transition at 13 mN/m. However, at 40-degrees-C the pressure-area curve for this lipid is characteristic for a monolayer in a liquid state. Mixed MAMGlcDAG-DGlcDAG and MGlcDAG-DGlcDAG monolayers showed no significant deviation from the additivity rule at 40-degrees-C. The area per acyl chain is nearly the same for MAMGlcDAG and MGlcDAG. Our study supports our previous results that aqueous dispersions of these lipids form non-lamellar, reversed aggregates.