ANTENATAL DIAGNOSIS OF HEMOGLOBINOPATHIES BY BIOREX CHROMATOGRAPHY OF HEMOGLOBIN

被引：13

作者：

BLOUQUIT, Y

BEUZARD, Y

VARNAVIDES, L

CHABRET, C

DUMEZ, Y

JOHN, PN

RODECK, C

WHITE, JM

机构：

[1] HOP HENRI MONDOR, INSERM, U91, UNITE RECH ANEMIES, F-94010 CRETEIL, FRANCE

[2] HOP HENRI MONDOR, BIOCHIM LAB, F-94010 CRETEIL, FRANCE

[3] MATERN PORT ROYAL, F-75014 PARIS, FRANCE

[4] UNIV LONDON KINGS COLL HOSP, SCH MED, DEPT OBSTET & GYNAECOL, LONDON SE5 8RX, ENGLAND

[5] UNIV LONDON KINGS COLL HOSP, SCH MED, DEPT HAEMATOL, LONDON SE5 8RX, ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1982年 / 50卷 / 01期

关键词：

D O I：

10.1111/j.1365-2141.1982.tb01885.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Biorex chromatography of Hb was compared to the standard chromatographic separation of radioactive globin chains in 60 [human] fetal blood samples obtained for the antenatal diagnosis of hemoglobinopathies. Biorex chromatography of Hb permitted 2 measurements, the optical density at 418 nm and the radioactivity incorporated into fetal and adult Hb. The 2 measurements were highly correlated (r2 = 0.96) and enabled a distinction between homozygous from heterozygous states of the diseases to be made, particularly in .beta. thalassemia. A single column was used for 50 analyses. This fast and very sensitive method is proposed for the antenatal diagnosis of hemoglobinopathies using fetal blood.

引用

页码：7 / 15

页数：9

共 10 条

[1] PRENATAL DIAGNOSIS OF HEMOGLOBINOPATHIES AND OTHER HEMATOLOGIC DISEASES
ALTER, BP
[J]. JOURNAL OF PEDIATRICS, 1979, 95 (04) : 501 - 513
[2] CONGOTE LF, 1981, BLOOD, V57, P353
[3] DUBART A, 1980, BLOOD, V56, P1092
[4] PRENATAL-DIAGNOSIS OF BETA-THALASSEMIA BY FETAL RED-CELL ENRICHMENT WITH NH4CL-NH4HCO3 DIFFERENTIAL LYSIS OF MATERNAL CELLS
FURBETTA, M
ANGIUS, A
XIMENES, A
TUVERI, T
ROSATELLI, C
SCALAS, MT
FAIS, R
CAO, A
ANGIONI, G
CAMINITI, F
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1980, 44 (03) : 441 - 450
[5] KAN YW, 1978, LANCET, V2, P910
[6] KAN YW, 1977, LANCET, V1, P269
[7] POLYMORPHISM OF DNA-SEQUENCE IN THE BETA-GLOBIN GENE REGION - APPLICATION TO PRENATAL-DIAGNOSIS OF BETA-0-THALASSEMIA IN SARDINIA
KAN, YW
LEE, KY
FURBETTA, M
ANGIUS, A
CAO, A
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1980, 302 (04) : 185 - 188
[8] MODEL FOR ANTENATAL DIAGNOSIS OF BETA-THALASSEMIA AND OTHER MONOGENIC DISORDERS BY MOLECULAR ANALYSIS OF LINKED DNA POLYMORPHISMS
LITTLE, PFR
ANNISON, G
DARLING, S
WILLIAMSON, R
CAMBA, L
MODELL, B
[J]. NATURE, 1980, 285 (5761) : 144 - 147
[9] HEMATOLOGICAL ASPECTS OF ANTENATAL DIAGNOSIS FOR THALASSEMIA IN BRITAIN
MATSAKIS, M
BERDOUKAS, VA
ANGASTINIOTIS, M
MOUZOURAS, M
IOANNOU, P
FERRARI, M
MODELL, B
FAIRWEATHER, DVI
WARD, RHT
LOUKOPOULOS, D
SAKARELLOU, N
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1980, 46 (02) : 185 - 197
[10] APPLICATION OF ENDONUCLEASE MAPPING TO ANALYSIS AND PRENATAL DIAGNOSIS OF THALASSEMIAS CAUSED BY GLOBIN-GENE DELETION
ORKIN, SH
ALTER, BP
ALTAY, C
MAHONEY, MJ
LAZARUS, H
HOBBINS, JC
NATHAN, DG
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1978, 299 (04) : 166 - 172

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