CHARACTERIZATION OF A NEW REVERSED-PHASE CHROMATOGRAPHIC COLUMN ON A 2-MU-M POROUS MICROSPHERICAL SILICA-GEL

The physical and chromatographic properties of a reversed-phase column based on a 2-mu m porous silica gel, TSKgel Super-ODS, were investigated. The Super-ODS column reveals higher column efficiencies (over 200 000 theoretical plates/m) at a higher flow-rate than that on a conventional column. High selectivity for a planar compound on Super-ODS implies that it possesses a polymeric ODS layer. Employing pure silica and a full end-capping procedure resulted in sharp peaks for basic and chelating compounds. To avoid the loss of column efficiency, the void volumes in an operating system such as connecting tubes, eel volume and sample volume, should be minimized as much as possible. The time constant of the detector also affected the column efficiency. The separation of peptides was achieved within 4 min.