INVITRO CLONAL PROPAGATION OF DIOECIOUS CARICA-PAPAYA

被引：52

作者：

REUVENI, O

SHLESINGER, DR

LAVI, U

机构：

[1] Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 20卷 / 01期

关键词：

endophytic contamination; micropropagation; papaya; rifampicin; tissue culture;

D O I：

10.1007/BF00034755

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl-1 rifampicin or by incorporating it at 50 mgl-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl-1 6-benzyladenine and 0.1 mgl-1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mgl-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl-1 kinetin and 0.05 mgl-1 naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0 mgl-1 indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist. © 1990 Kluwer Academic Publishers.

引用

页码：41 / 46

页数：6

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