ANALYSIS OF AN ANAEROBICALLY INDUCED PROMOTER FOR THE COBALAMIN BIOSYNTHETIC GENES IN SALMONELLA-TYPHIMURIUM

被引：11

作者：

RICHTERDAHLFORS, AA ^{[1
]}

ANDERSSON, DI ^{[1
]}

机构：

[1] UNIV UPPSALA, CTR BIOMED, DEPT MICROBIOL, BOX 581, S-75123 UPPSALA, SWEDEN

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00780.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified an anaerobically induced promoter for the cobalamin biosynthetic (cob) genes. In a plasmid the Cob promoter showed two of the three types of control of the intact chromosomal Cob operon: anaerobic induction and cAMP stimulation. Cobalamin repression was observed only in promoter fragments which included sequences far downstream of the transcription start site, suggesting that this control is post-transcriptional. One anaerobically induced transcript was identified and its 5' end was determined. Deletion mapping showed that 60 nucleotides upstream of the start site were sufficient for anaerobic synthesis of this transcript. Upstream of the transcription start site a putative sigma-70-dependent -10 recognition sequence was identified; however, no consensus -35 region was observed.

引用

页码：1337 / 1345

页数：9

共 47 条

[1] REDOX REGULATION OF THE GENES FOR COBINAMIDE BIOSYNTHESIS IN SALMONELLA-TYPHIMURIUM [J].

ANDERSSON, DI ;

ROTH, JR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (12) :6734-6739

[2] MUTATIONS AFFECTING REGULATION OF COBINAMIDE BIOSYNTHESIS IN SALMONELLA-TYPHIMURIUM [J].

ANDERSSON, DI ;

ROTH, JR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (12) :6726-6733

[3] AN OVERLAP BETWEEN OSMOTIC AND ANAEROBIC STRESS RESPONSES - A POTENTIAL ROLE FOR DNA SUPERCOILING IN THE COORDINATE REGULATION OF GENE-EXPRESSION [J].

BHRIAIN, NN ;

DORMAN, CJ ;

HIGGINS, CF .

MOLECULAR MICROBIOLOGY, 1989, 3 (07) :933-942

[4] INVOLVEMENT OF THE NTRA GENE-PRODUCT IN THE ANAEROBIC METABOLISM OF ESCHERICHIA-COLI [J].

BIRKMANN, A ;

SAWERS, RG ;

BOCK, A .

MOLECULAR & GENERAL GENETICS, 1987, 210 (03) :535-542

[5]

BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376

[6] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].

CASADABAN, MJ ;

COHEN, SN .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :179-207

[7] EVIDENCE FOR B12-DEPENDENT ENZYME ETHANOLAMINE DEAMINASE IN SALMONELLA [J].

CHANG, GW ;

CHANG, JT .

NATURE, 1975, 254 (5496) :150-151

[8] MUTATION LEADING TO TOTAL LACK OF NITRITE REDUCTASE-ACTIVITY IN ESCHERICHIA-COLI-K-12 [J].

CHIPPAUX, M ;

GIUDICI, D ;

ABOUJAOUDE, A ;

CASSE, F ;

PASCAL, MC .

MOLECULAR & GENERAL GENETICS, 1978, 160 (02) :225-229

[9] NITRITE REDUCTASE DEFICIENT MUTANTS OF ESCHERICHIA-COLI K12 [J].

COLE, JA ;

WARD, FB .

JOURNAL OF GENERAL MICROBIOLOGY, 1973, 76 (MAY) :21-29

[10]

Davis RW, 1980, ADV BACTERIAL GENETI

← 1 2 3 4 5 →