MORPHINE AMPLIFIES HIV-1 EXPRESSION IN CHRONICALLY INFECTED PROMONOCYTES COCULTURED WITH HUMAN BRAIN-CELLS

被引：106

作者：

PETERSON, PK

GEKKER, G

HU, SX

ANDERSON, WR

KRAVITZ, F

PORTOGHESE, PS

BALFOUR, HH

CHAO, CC

机构：

[1] UNIV MINNESOTA,SCH MED,MINNEAPOLIS,MN 55455

[2] UNIV MINNESOTA,DEPT MED CHEM,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1994年 / 50卷 / 02期

关键词：

MORPHINE; HUMAN IMMUNODEFICIENCY VIRUS-1; HUMAN BRAIN CELL CULTURE; TUMOR NECROSIS- FACTOR-ALPHA; MICROGLIA;

D O I：

10.1016/0165-5728(94)90043-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous studies have shown that morphine promotes the replication of human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear cell cocultures. In the present study, we tested the hypothesis that morphine would amplify HIV-1 expression in the chronically infected promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated human fetal brain cells. Marked upregulation of HIV-1 expression was observed in these cocultures (quantified by measurement of HIV-1 p24 antigen levels in supernatants), and treatment of brain cells with morphine resulted in a bell-shaped dose-dependent enhancement of viral expression. The mechanism of morphine's amplifying effect appears to be opioid receptor-mediated and to involve enhanced production of tumor necrosis factor-alpha by microglial cells.

引用

页码：167 / 175

页数：9

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