PREPARATION OF 14,38-BIS-[S-CARBAMIDOMETHYL]-(BASIC TRYPSIN INHIBITOR) POSSESSING FULL BIOLOGICAL ACTIVITY

被引:44
作者
LIU, WK
MEIENHOFER, J
机构
[1] Children's Cancer Research Foundation, The Children's Hospital Medical Center Boston
[2] Harvard Medical School, Boston
关键词
D O I
10.1016/0006-291X(68)90500-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dithiothreitol reduced selectively the disulfide bond linking the half cystine residues 14 and 38 of bovine basic trypsin inhibitor in the absence of urea at pH 8.2. Iodoacetamide treatment afforded 14, 38-bis-[S-carbamidomethyl]-(basic trypsin inhibitor) possessing full biological activity. © 1968.
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页码:467 / +
页数:1
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