STUDIES OF ELECTROIMMUNODIFFUSION - IMMUNOCHEMICAL QUANTITATION OF PROTEINS IN DILUTE SOLUTIONS

The behavior of serum albumin, IgG, IgM, IgA and salivary IgA was studied in the recently-introduced quantiative technique of electroimmunodifussion. This method by combining the modes of immunodiffusion through antibody-containing agar and electrophoresis of antigen achieved performance advantages over radial immunodiffusion in the quantitation of protein antigens, particularly when in dilute solutions. From the results obtained with these standard antigens of varying molecular size and electrophoretic mobility, principles have been derived which should allow the adaptation of this method to the measurement of any soluble protein. © 1969.