DETECTION OF H-2DB ANTIGEN ON OSMOTIC SHOCK-TREATED FRIEND-LEUKEMIA VIRUS-PARTICLES

Antisera specific for either H-2Kb, H-2Db, H-2Kk or H-2Dk antigenic determinants were examined for their capacity to neutralize Friend virus (FV) collected from the serum of infected H-2b/H-2k heterozygous mice. Neutralizing activity was detected (1) only with anti-H-2Db antisera, (2) only when the surface of virus particles had been mildly deranged by osmotic shock treatment and (3) only in the assay for the defective spleen focus-forming virus component of FV. © 1979 Springer-Verlag New York Inc.