NOVEL DIRECT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF SALICYLATE GLUCURONIDE CONJUGATES IN HUMAN URINE

被引：12

作者：

SHEN, JJ ^{[1
]}

WANWIMOLRUK, S ^{[1
]}

ROBERTS, MS ^{[1
]}

机构：

[1] UNIV OTAGO,SCH MED,DEPT PHARM,POB 913,DUNEDIN,NEW ZEALAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80392-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel direct high-performance liquid chromatographic (HPLC) assay for the simultaneous determination of three salicylate glucuronide conjugates and other salicylate metabolites in human urine has been developed. Salicylate glucuronide conjugates were purified by HPLC from the urine of a volunteer after oral administration of aspirin and identified by selective hydrolysis with beta-glucuronidase and with sodium hydroxide. This method gave high reproducibility with coefficients of variation less than 10%. The total urinary recovery of salicylic acid after a single 1.2-g dose of soluble aspirin was greater than 90%. This assay has been successfully used to re-evaluated the capacity-limited pharmacokinetics of salicylic acid in humans.

引用

页码：309 / 320

页数：12

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