CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE ENGRAILED HOMEODOMAIN AND OF AN ENGRAILED HOMEODOMAIN DNA COMPLEX

被引:15
作者
LIU, BS [1 ]
KISSINGER, CR [1 ]
PABO, CO [1 ]
MARTINBLANCO, E [1 ]
KORNBERG, TB [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143
关键词
D O I
10.1016/0006-291X(90)91385-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The homeodomain from the engrailed protein of Drosophila has been crystallized from ammonium phosphate at pH 6.8. The crystals form in space group P6122 (or P6522), with cell dimensions a = b = 44.8 Å and c = 118.2 Å. These crystals diffract to 1.8 Å resolution. A complex containing the engrailed homeodomain and a duplex DNA site also has been crystallized. The cocrystals form in space group C2 with a = 131.2 Å, b = 45.5 Å, c = 72.9 Å and β = 119.0°. These crystals diffract to 2.6 Å resolution. © 1990.
引用
收藏
页码:257 / 259
页数:3
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