PRODUCTION AND LIGAND-BINDING CHARACTERISTICS OF THE SOLUBLE FORM OF MURINE ERYTHROPOIETIN RECEPTOR

被引：55

作者：

NAGAO, M ^{[1
]}

MASUDA, S ^{[1
]}

ABE, S ^{[1
]}

UEDA, M ^{[1
]}

SASAKI, R ^{[1
]}

机构：

[1] SNOW BRAND MILK PROD CO LTD, LIFE SCI RES INST, TOCHIGI 32905, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91139-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant soluble form (sEPO-R) of erythropoietin (EPO) receptor (EPO-R) was produced by Chinese hamster ovary cells and isolated in high yield with the EPO-fixed gel. Ligand binding assays were done using three methods; precipitation of sEPO-R radiolabeled EPO complex and competition of sEPO-R for the binding of radiolabeled EPO with the cellular EPO-R. The results showed a Kd of 17 nM which was much lower than those for cellular EPO-R. One N-glycosylation site exists in sEPO-R but the glycosylation did not affect the binding affinity to EPO. A complex with a molecular size that corresponded to a 1:1 complex of EPO and sEPO-R was detected. © 1992.

引用

页码：888 / 897

页数：10

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