EXPRESSION OF PHOSPHATIDYLSERINE-DEPENDENT ANTIGENS ON THE SURFACE OF DIFFERENTIATING BEWO HUMAN CHORIOCARCINOMA CELLS

被引:34
作者
ROTE, NS
CHANG, J
KATSURAGAWA, H
NG, AK
LYDEN, TW
MORI, T
机构
[1] WRIGHT STATE UNIV,SCH MED,DEPT OBSTET & GYNECOL,DAYTON,OH
[2] UNIV SO MAINE,DEPT APPL SCI MED,PORTLAND,ME 04103
[3] KYOTO UNIV,FAC MED,DEPT OBSTET & GYNECOL,KYOTO,JAPAN
来源
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1995年 / 33卷 / 01期
关键词
ANTIPHOSPHOLIPID ANTIBODY; PHOSPHATIDYLSERINE; BEWO; CARDIOLIPIN;
D O I
10.1111/j.1600-0897.1995.tb01147.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
PROBLEM: Antiphospholipid antibodies (aPLs) are associated with pregnancy loss, pregnancy-induced hypertension, and intrauterine growth retardation. We have previously reported that phosphatidylserine (PS)-dependent antigens are expressed in formalin-fixed cells concurrent with differentiation in a choriocarcinoma model (BeWo) of cytotrophoblast, That study, however, could not differentiate between cytoplasmic or surface antigen expression. METHOD: Three monoclonal aPLs that differentiate between PS- and cardiolipin (CL) dependent antigens were reacted with BeWo, with or without forskolin activation, before fixation, and antibody binding was evaluated by immunoperoxidase techniques. RESULTS: Activation with forskolin induced a PS-dependent antigenic determinant on the surface on BeWo cells. CL-reactive monoclonal antibodies did not react with the cell surface, whether forskolin treated or not. CONCLUSION: These observations demonstrate that a PS-dependent antigen is expressed on the surface of a model of differentiating cytotrophoblastic cells and should be accessible in vivo to circulating aPLs.
引用
收藏
页码:114 / 121
页数:8
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