SHORT INESCAPABLE STRESS PRODUCES LONG-LASTING CHANGES IN THE BRAIN-PITUITARY-ADRENAL AXIS OF ADULT MALE-RATS

被引:169
作者
VANDIJKEN, HH
DEGOEIJ, DCE
SUTANTO, W
MOS, J
DEKLOET, E
TILDERS, FJH
机构
[1] FREE UNIV AMSTERDAM,FAC MED,DEPT PHARMACOL,VAN DER BOECHORST 7,1081 BT AMSTERDAM,NETHERLANDS
[2] SOLVAY DUPHAR BV,CNS PHARMACOL,WEESP,NETHERLANDS
[3] LEIDEN UNIV,CTR BIOPHARMACEUT SCI,DIV MED PHARMACOL,2300 RA LEIDEN,NETHERLANDS
关键词
FOOTSHOCKS; STRESS; HIPPOCAMPUS; MINERALOCORTICOID RECEPTOR; GLUCOCORTICOID RECEPTOR; VASOPRESSIN; CRF; ACTH; CORTICOSTERONE; BEHAVIOR; RAT;
D O I
10.1159/000126512
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recently, we reported that rats exposed to a single and short session of inescapable footshocks showed alterations in behavioural response to environmental stimuli which developed progressively over a week and remained present for at least 28 days. The aim of the present study was to investigate whether these behavioural changes were accompanied by alterations in the brain-pituitary-adrenal axis. Male Wistar rats were subjected to 10 inescapable footshocks (S) of 6 s duration and 1 mA intensity during a period of 15 min. Control rats (C) were placed in the shock apparatus for 15 min without receiving shocks. The effects of these experimental procedures were studied 14 days later. Exposure to shocks did not affect basal plasma levels of adrenocorticotropic hormone (ACTH) and corticosterone (CORT). However, the novelty-induced ACTH response was increased in S rats as compared to C rats whereas the CORT response did not differ between C and S rats. The ACTH content of the anterior pituitary gland and adrenal weight were not affected by exposure to inescapable footshocks 14 days earlier. Quantitative immunocytochemistry of vasopressin (AVP) and corticotropin-releasing factor (CRF) in the external zone of the median eminence showed that prior footshock exposure increased the AVP(i) stores to 167% as compared to C rats, whereas CRF(i) content was not changed. In addition, S rats showed increased mineralocorticoid (MR) and glucocorticoid (GR) receptor binding capacity in the hippocampus as compared to C rats, whereas affinities were not affected. We conclude that a single and short session of inescapable footshocks has longlasting effects on brain-pituitary-adrenal functioning concomitant with behavioural alterations.
引用
收藏
页码:57 / 64
页数:8
相关论文
共 46 条
[1]   EVIDENCE THAT THE PITUITARY-ADRENAL AXIS DOES NOT CROSS-ADAPT TO STRESSORS - COMPARISON TO OTHER PHYSIOLOGICAL VARIABLES [J].
ARMARIO, A ;
HIDALGO, J ;
GIRALT, M .
NEUROENDOCRINOLOGY, 1988, 47 (03) :263-267
[2]  
ARMARIO A, 1985, LIFE SCI, V36, P1085
[4]   COLOCALIZATION OF PEPTIDE HISTIDINE ISOLEUCINE AMINE AND CORTICOTROPIN-RELEASING FACTOR IMMUNOREACTIVITY IN NEURONS OF THE RAT HYPOTHALAMUS - A SURPRISING ARTIFACT [J].
BERKENBOSCH, F ;
LINTON, EA ;
TILDERS, FJH .
NEUROENDOCRINOLOGY, 1986, 44 (03) :338-346
[5]   REGULATION OF BASAL ACTH-SECRETION BY CORTICOSTERONE IS MEDIATED BY BOTH TYPE-I (MR) AND TYPE-II (GR) RECEPTORS IN RAT-BRAIN [J].
BRADBURY, MJ ;
AKANA, SF ;
CASCIO, CS ;
LEVIN, N ;
JACOBSON, L ;
DALLMAN, MF .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1991, 40 (1-3) :133-142
[6]   PITUITARY ADRENOCORTICAL-RESPONSE TO CHRONIC INTERMITTENT STRESS [J].
BURCHFIELD, SR ;
WOODS, SC ;
ELICH, MS .
PHYSIOLOGY & BEHAVIOR, 1980, 24 (02) :297-302
[7]   PRIOR STRESS ATTENUATES THE ANALGESIC RESPONSE BUT SENSITIZES THE CORTICOSTERONE AND CORTICAL DOPAMINE RESPONSES TO STRESS 10 DAYS LATER [J].
CAGGIULA, AR ;
ANTELMAN, SM ;
AUL, E ;
KNOPF, S ;
EDWARDS, DJ .
PSYCHOPHARMACOLOGY, 1989, 99 (02) :233-237
[8]  
CHAPPELL PB, 1986, J NEUROSCI, V6, P2908
[9]   REPEATED STRESS-INDUCED ACTIVATION OF CORTICOTROPIN-RELEASING FACTOR NEURONS ENHANCES VASOPRESSIN STORES AND COLOCALIZATION WITH CORTICOTROPIN-RELEASING FACTOR IN THE MEDIAN-EMINENCE OF RATS [J].
DE GOEIJ, DCE ;
KVETNANSKY, R ;
WHITNALL, MH ;
JEZOVA, D ;
BERKENBOSCH, F ;
TILDERS, FJH .
NEUROENDOCRINOLOGY, 1991, 53 (02) :150-159
[10]   CHRONIC PSYCHOSOCIAL STRESS ENHANCES VASOPRESSIN, BUT NOT CORTICOTROPIN-RELEASING FACTOR, IN THE EXTERNAL ZONE OF THE MEDIAN-EMINENCE OF MALE-RATS - RELATIONSHIP TO SUBORDINATE STATUS [J].
DEGOEIJ, DCE ;
DIJKSTRA, H ;
TILDERS, FJH .
ENDOCRINOLOGY, 1992, 131 (02) :847-853