CONSERVATION OF THE SIZES OF 53 INTRONS AND OVER 100 INTRONIC SEQUENCES FOR THE BINDING OF COMMON TRANSCRIPTION FACTORS IN THE HUMAN AND MOUSE GENES FOR TYPE-II PROCOLLAGEN (COL2A1)

被引：22

作者：

ALAKOKKO, L

KVIST, AP

METSARANTA, M

KIVIRIKKO, KI

DECROMBRUGGHE, B

PROCKOP, DJ

VUORIO, E

机构：

[1] UNIV OULU,BIOCTR,COLLAGEN RES UNIT,OULU,FINLAND

[2] UNIV OULU,DEPT MED BIOCHEM,OULU,FINLAND

[3] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,JEFFERSON INST MOLEC MED,DEPT BIOCHEM & MOLEC BIOL,PHILADELPHIA,PA 19107

[4] UNIV TURKU,DEPT MED BIOCHEM,TURKU,FINLAND

[5] UNIV TURKU,DEPT MOLEC BIOL,TURKU,FINLAND

[6] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,HOUSTON,TX 77030

来源：

BIOCHEMICAL JOURNAL | 1995年 / 308卷

关键词：

D O I：

10.1042/bj3080923

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over 11000 bp of previously undefined sequences of the human COL2A1 gene were defined. The results made it possible to compare the intron structures of a highly complex gene from man and mouse. Suprisingly, the sizes of the 53 introns of the two genes were highly conserved with a mean difference of 13%. After alignment of the sequences, 69% of the intron sequences were identical. The introns contained consensus sequences for the binding of over 100 different transcription factors that were conserved in the introns of the two genes. The first intron of the gene contained 80 conserved consensus sequences and the remaining 52 introns of the gene contained 106 conserved sequences for the binding of transcription factors. The 5'-end of intron 2 in both genes had a potential for forming a stem loop in RNA transcripts.

引用

页码：923 / 929

页数：7

共 38 条

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