STRUCTURE OF THE GENE ENCODING CONCANAVALIN-A FROM CANAVALIA-GLADIATA AND ITS EXPRESSION IN ESCHERICHIA-COLI-CELLS

被引：42

作者：

YAMAUCHI, D

MINAMIKAWA, T

机构：

[1] Department of Biology, Tokyo Metropolitan University, Setagaya-ku, Tokyo, 158, Fukazawa

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

(Canavalia gladiata; Escherichia coli); Carbohydrate binding activity; Concanavalin A; Gene structure; Legume lectin; Peptide ligation; Processing;

D O I：

10.1016/0014-5793(90)80083-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata. The sequence covers the whole transcribed region as well as the 5'- and 3'-untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G-50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation. © 1990.

引用

页码：127 / 130

页数：4

共 17 条

[1] POSTTRANSLATIONAL PROCESSING OF CONCANAVALIN-A PRECURSORS IN JACKBEAN COTYLEDONS [J].