MISSENSE MUTATION IN THE GENE CODING FOR RIBOSOMAL PROTEIN-S 17 (RPSQ) LEADING TO RIBOSOMAL ASSEMBLY DEFECTIVITY IN ESCHERICHIA-COLI

被引:24
作者
HERZOG, A [1 ]
YAGUCHI, M [1 ]
CABEZON, T [1 ]
CORCHUELO, MC [1 ]
PETRE, J [1 ]
BOLLEN, A [1 ]
机构
[1] NATL RES COUNCIL CANADA, DIV BIOL SCI, OTTAWA K1A 0R6, ONTARIO, CANADA
来源
MOLECULAR AND GENERAL GENETICS | 1979年 / 171卷 / 01期
关键词
D O I
10.1007/BF00274010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The conditionally lethal mutation, 286lmis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal particles at 42°C. The strain carrying the mutation has an altered S17 ribosomal protein; the mutational alteration involves a replacement of serine by phenylalanine in protein S17. Spontaneous reversion to temperature independence can restore the normal assembly in vivo of 30S ribosomal subunits at 42°C and the normal chromatographical sehaviour of the S17 ribosomal protein in vitro. We conclude therefore that the 2861mis mutation affects the structural gene for protein S17 (rpsQ). © 1979 Springer-Verlag.
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页码:15 / 22
页数:8
相关论文
共 33 条
[1]   RECALIBRATED LINKAGE MAP OF ESCHERICHIA-COLI K-12 [J].
BACHMANN, BJ ;
LOW, KB ;
TAYLOR, AL .
BACTERIOLOGICAL REVIEWS, 1976, 40 (01) :116-167
[2]   LOCALIZED MUTAGENESIS OF AROE-STRA SECTION OF ESCHERICHIA-COLI CHROMOSOME CODING FOR RIBOSOMAL-PROTEINS [J].
BERGER, I ;
GEYL, D ;
BOCK, A ;
STOFFLER, G ;
WITTMANN, HG .
MOLECULAR & GENERAL GENETICS, 1975, 141 (03) :207-211
[3]   ALTERATION OF RIBOSOMAL-PROTEIN S17 BY MUTATION LINKED TO NEAMINE RESISTANCE IN ESCHERICHIA-COLI .1. GENERAL PROPERTIES OF NEAA MUTANTS [J].
BOLLEN, A ;
CABEZON, T ;
WILDE, MD ;
VILLARROEL, R ;
HERZOG, A .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 99 (04) :795-+
[4]  
BOLLEN A, 1970, FEBS LETT, V6, P119
[5]   CHEMICAL STUDIES ON METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA-COLI [J].
BRUTON, CJ ;
HARTLEY, BS .
JOURNAL OF MOLECULAR BIOLOGY, 1970, 52 (02) :165-&
[6]   RIBOSOMAL ASSEMBLY DEFICIENCY IN AN ESCHERICHIA-COLI THERMOSENSITIVE MUTANT HAVING AN ALTERED L24 RIBOSOMAL-PROTEIN [J].
CABEZON, T ;
HERZOG, A ;
PETRE, J ;
YAGUCHI, M ;
BOLLEN, A .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 116 (03) :361-374
[7]   CHARACTERIZATION OF CONDITIONALLY LETHAL MUTATIONS AFFECTING RIBOSOMAL-PROTEINS IN ESCHERICHIA-COLI [J].
CABEZON, T ;
DELCUVE, G ;
HERZOG, A ;
PETRE, J ;
BOLLEN, A .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1977, 5 (04) :958-959
[8]   MAPPING OF NEAMINE RESISTANCE - IDENTIFICATION OF 2 GENETIC LOCI, NEA-A AND NEA-B [J].
CANNON, M ;
CABEZON, T ;
BOLLEN, A .
MOLECULAR & GENERAL GENETICS, 1974, 130 (04) :321-326
[9]   SEQUENCE DETERMINATION OF A PROTEIN IN A MICRO SCALE - SEQUENCE-ANALYSIS OF RIBOSOMAL-PROTEIN L34 OF ESCHERICHIA-COLI [J].
CHEN, R .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1976, 357 (06) :873-886
[10]   AMBER MUTATIONS IN ESCHERICHIA-COLI ESSENTIAL GENES - ISOLATION OF MUTANTS AFFECTED IN RIBOSOMES [J].
DELCUVE, G ;
CABEZON, T ;
GHYSEN, A ;
HERZOG, A ;
BOLLEN, A .
MOLECULAR & GENERAL GENETICS, 1977, 157 (02) :149-153