MEASUREMENT OF DNA LENGTH BY GEL-ELECTROPHORESIS

被引：335

作者：

SOUTHERN, EM

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90235-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plotting fragment length against reciprocal of mobility gives a straight line over a wider range than the conventional semilogarithmic plot. Curvature can be removed by a simple correction. A method is also given for determining molecular weights from mobilities by direct calculation. © 1979.

引用

页码：319 / 323

页数：5

共 7 条

[1] ROLE OF MOLECULAR CONFORMATION IN DETERMINING ELECTROPHORETIC PROPERTIES OF POLYNUCLEOTIDES IN AGAROSE-ACRYLAMIDE COMPOSITE GELS
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[3] NEW METHOD FOR SEQUENCING DNA
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[4] ANALYSIS OF RESTRICTION FRAGMENTS OF T7 DNA AND DETERMINATION OF MOLECULAR-WEIGHTS BY ELECTROPHORESIS IN NEUTRAL AND ALKALINE GELS
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[5] CLONING OF YEAST RIBOSOMAL DNA REPEAT UNIT IN SSTI AND HINDIII LAMBDA VECTORS USING GENETIC AND PHYSICAL SIZE SELECTIONS
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[J]. JOURNAL OF MOLECULAR BIOLOGY, 1978, 123 (03) : 371 - 386
[6] PREPARATIVE GEL-ELECTROPHORESIS APPARATUS FOR LARGE-SCALE SEPARATIONS
SOUTHERN, EM
[J]. ANALYTICAL BIOCHEMISTRY, 1979, 100 (02) : 304 - 318
[7] NUCLEOTIDE-SEQUENCE OF SV40 DNA RESTRICTION FRAGMENT HIND C-HAP2
VANHEUVERSWYN, H
VANDEVOORDE, A
FIERS, W
[J]. NUCLEIC ACIDS RESEARCH, 1977, 4 (04) : 1015 - 1024

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