DNA PROBES FOR DETECTION OF COPPER RESISTANCE GENES IN XANTHOMONAS-CAMPESTRIS PV VESICATORIA

被引：29

作者：

GARDE, S ^{[1
]}

BENDER, CL ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,DEPT PLANT PATHOL,STILLWATER,OK 74078

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 08期

关键词：

D O I：

10.1128/AEM.57.8.2435-2439.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The copper resistance (Cu(r)) genes encoded on pXV10A, a 190-kb plasmid in Xanthomonas campestris pv. vesicatoria XV10, were isolated on a 44-kb cosmid clone designated pCuR1. Tn5 mutagenesis of pCuR1 indicated that a 4.0-kb region was required for copper resistance. Three restriction fragments located within the 4.0-kb region demonstrated high specificity for the Cu(r) genes present in X. campestris pv. vesicatoria and will be useful in monitoring the presence of these genes in the environment.

引用

页码：2435 / 2439

页数：5

共 22 条

[1] COPPER TOLERANCE AND ZINC SENSITIVITY OF MEXICAN STRAINS OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA, CAUSAL AGENT OF BACTERIAL SPOT OF PEPPER [J].

ADASKAVEG, JE ;

HINE, RB .

PLANT DISEASE, 1985, 69 (11) :993-996

[2] LINKAGE MAP OF ESCHERICHIA-COLI-K-12, EDITION-7 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1983, 47 (02) :180-230

[3] MOLECULAR-CLONING OF COPPER RESISTANCE GENES FROM PSEUDOMONAS-SYRINGAE PV TOMATO [J].

BENDER, CL ;

COOKSEY, DA .

JOURNAL OF BACTERIOLOGY, 1987, 169 (02) :470-474

[4] INDIGENOUS PLASMIDS IN PSEUDOMONAS-SYRINGAE PV TOMATO - CONJUGATIVE TRANSFER AND ROLE IN COPPER RESISTANCE [J].

BENDER, CL ;

COOKSEY, DA .

JOURNAL OF BACTERIOLOGY, 1986, 165 (02) :534-541

[5] CONSERVATION OF PLASMID DNA-SEQUENCES IN CORONATINE-PRODUCING PATHOVARS OF PSEUDOMONAS-SYRINGAE [J].

BENDER, CL ;

YOUNG, SA ;

MITCHELL, RE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) :993-999

[6] CHARACTERIZATION OF PXV10A, A COPPER RESISTANCE PLASMID IN XANTHOMONAS-CAMPESTRIS PV VESICATORIA [J].

BENDER, CL ;

MALVICK, DK ;

CONWAY, KE ;

GEORGE, S ;

PRATT, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (01) :170-175

[7] PLASMID-DETERMINED COPPER RESISTANCE IN PSEUDOMONAS-SYRINGAE FROM IMPATIENS [J].

COOKSEY, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (01) :13-16

[8] COPPER RESISTANCE GENE HOMOLOGS IN PATHOGENIC AND SAPROPHYTIC BACTERIAL SPECIES FROM TOMATO [J].

COOKSEY, DA ;

AZAD, HR ;

CHA, JS ;

LIM, CK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (02) :431-435

[9]

CORSA JH, 1981, PLASMIDS, P266

[10]

CROUSE GF, 1983, METHOD ENZYMOL, V101, P78

← 1 2 3 →