RAPID AND SIMPLE ISOLATION OF PURE PHOTOSYSTEM-II CORE AND REACTION CENTER PARTICLES FROM SPINACH

Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-beta-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.