BLEOMYCIN RESISTANCE AS A SELECTABLE MARKER FOR TRANSFORMATION OF THE EUKARYOTE, DICTYOSTELIUM-DISCOIDEUM

被引：6

作者：

CHANG, ACM

HALL, RM

WILLIAMS, KL

机构：

[1] MACQUARIE UNIV,SCH BIOL SCI,SYDNEY,NSW 2109,AUSTRALIA

[2] CSIRO,DIV BIOMOLEC ENGN,N RYDE,NSW 2113,AUSTRALIA

来源：

GENE | 1991年 / 107卷 / 01期

关键词：

EXTRACHROMOSOMAL PLASMIDS; COTRANSLATION; BICISTRONIC MESSAGE; PHLEOMYCIN; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(91)90312-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An expression cassette was constructed, which has the bacterial bleomycin (Bm) resistance-encoding gene (ble) fused to the Dictyostelium discoideum actin-6 promoter, with a segment of 3'-flanking DNA from the actin-8-encoding gene placed downstream from the ble gene to serve as a transcription terminator. Plasmid pMUW161, which contains this cassette and the D. discoideum plasmid Ddp2 origin of DNA replication, transformed D. discoideum with high efficiency under Bm selection. Hence, this construct is useful as a dominant selectable marker for D. discoideum.

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收藏

页码：165 / 170

页数：6

相关论文

共 29 条

[1] OPTIMIZED VECTORS AND SELECTION FOR TRANSFORMATION OF NEUROSPORA-CRASSA AND ASPERGILLUS-NIDULANS TO BLEOMYCIN AND PHLEOMYCIN RESISTANCE [J].

AUSTIN, B ;

HALL, RM ;

TYLER, BM .

GENE, 1990, 93 (01) :157-162

[2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[3] COMPLEMENTATION OF A DICTYOSTELIUM-DISCOIDEUM THYMIDYLATE SYNTHASE MUTATION WITH THE MOUSE GENE PROVIDES A NEW SELECTABLE MARKER FOR TRANSFORMATION [J].

CHANG, ACM ;

WILLIAMS, KL ;

WILLIAMS, JG ;

CECCARELLI, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (10) :3655-3661

[4] IDENTIFICATION OF THE ORIGIN OF REPLICATION OF THE EUKARYOTE DICTYOSTELIUM-DISCOIDEUM NUCLEAR PLASMID DDP2 [J].

CHANG, ACM ;

SLADE, MB ;

WILLIAMS, KL .

PLASMID, 1990, 24 (03) :208-217

[5] IDENTIFICATION OF A TN5 DETERMINANT CONFERRING RESISTANCE TO PHLEOMYCINS, BLEOMYCINS, AND TALLYSOMYCINS [J].

COLLIS, CM ;

HALL, RM .

PLASMID, 1985, 14 (02) :143-151

[6] DISRUPTION OF THE DICTYOSTELIUM MYOSIN HEAVY-CHAIN GENE BY HOMOLOGOUS RECOMBINATION [J].

DELOZANNE, A ;

SPUDICH, JA .

SCIENCE, 1987, 236 (4805) :1086-1091

[7] MOLECULAR COMPLEMENTATION OF A GENETIC-MARKER IN DICTYOSTELIUM USING A GENOMIC DNA LIBRARY [J].

DYNES, JL ;

FIRTEL, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :7966-7970

[8] HYGROMYCIN RESISTANCE AS A SELECTABLE MARKER IN DICTYOSTELIUM-DISCOIDEUM [J].

EGELHOFF, TT ;

BROWN, SS ;

MANSTEIN, DJ ;

SPUDICH, JA .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (05) :1965-1968

[9] PHLEOMYCIN RESISTANCE ENCODED BY THE BLE GENE FROM TRANSPOSON TN 5 AS A DOMINANT SELECTABLE MARKER IN SACCHAROMYCES-CEREVISIAE [J].

GATIGNOL, A ;

BARON, M ;

TIRABY, G .

MOLECULAR & GENERAL GENETICS, 1987, 207 (2-3) :342-348

[10] THE TRANSPOSON-TN5 CARRIES A BLEOMYCIN-RESISTANCE DETERMINANT [J].

GENILLOUD, O ;

GARRIDO, MC ;

MORENO, F .

GENE, 1984, 32 (1-2) :225-233