PREPARATION OF A HYBRID OF FRAGMENT FAB-' OF ANTIBODY AND FRAGMENT A OF DIPHTHERIA-TOXIN AND ITS CYTOTOXICITY

被引：52

作者：

MASUHO, Y

HARA, T

NOGUCHI, T

机构：

[1] Teijin Institute for Biomedical Research Asahigaoka, Hino, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91627-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fragment A of diphtheria toxin was linked to the fragment Fab' of Ig from rabbit antiserum against L1210 cells by the reaction of Fab' with the S-sulfonated fragment A and purified by chromatography on Sephadex G 150. The hybrid thus prepared showed a high cytotoxicity in vitro to L1210 cells which are insensitive to diphtheria toxin itself. © 1979.

引用

页码：320 / 326

页数：7

共 15 条

[1]

CAMPBELL DH, 1967, METHODS IMMUNOLOGY I, V1, P335

[2]

COLLIER RJ, 1971, J BIOL CHEM, V246, P1496

[3] NORMAL DISTRIBUTION, PATCHING AND CAPPING OF LYMPHOCYTE SURFACE IMMUNOGLOBULIN STUDIED BY ELECTRON-MICROSCOPY [J].