PATHOLOGICAL-CHANGES IN BOVINE MAMMARY-GLANDS FOLLOWING INTRAMAMMARY INFUSION OF RECOMBINANT INTERLEUKIN-2

Eighteen lactating dairy cows were used to evaluate the physiological response of mammary glands to increasing doses of recombinant bovine interleukin-2. Right front and rear quarters were intramammarily infused with five different doses (.1 to 100-mu-g per quarter) of interleukin-2 as either a single or multiple treatment. Left front and rear quarters were intramammarily infused with a saline placebo and served as within-animal controls. Milk secretion samples for compositional analysis were collected from each quarter prior to infusion and at 12, 24, 36, and 48 h following infusion. Animals were slaughtered by exsanguination immediately following the 48-h sampling period, and mammary gland tissue was obtained for morphometric analysis. No changes in milk composition were observed between control quarters and those infused with up to 10-mu-g of interleukin-2 per quarter, administered as either a single or multiple treatment. Quarters infused with a single 100-mu-g dose of interleukin-2 or three consecutive doses of 25 and 100-mu-g of interleukin-2 had significantly lower lactose concentrations; there was a concomitant increase in bovine serum albumin, pH, and SCC compared with preinfusion concentrations or with control quarters. Morphometric analysis of tissue demonstrated an increase in stroma, a decrease in lumenal area, and a marked increase in the number of infiltrating leukocytes in those quarters infused with the higher doses of interleukin-2. Results suggest that interleukin-2 can be intramammarily infused at doses as high as 10-mu-g per quarter without adversely affecting milk quality or normal mammary gland function.