STIMULATION OF ERYTHROMYCIN A YIELD BY INTEGRATION OF A CHROMOSOMAL DNA FRAGMENT INCLUDING THE ERYC1 GENE INTO THE CHROMOSOME OF SACCHAROPOLYSPORA-ERYTHRAEA

被引：5

作者：

HANEL, F

SCHUMANN, G

FIEDLER, G

KRUGEL, H

机构：

[1] Hans Knöll Institute for Natural Product Research, Jena, 0-6900

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 5.2 kbp chromosomal DNA fragment including the eryC1 gene was integrated along with vector DNA into the chromosome of a S. erythraea wild-type strain. The erythromycin A production of transformants in the presence of thiostrepton was two to three times higher in comparison with the non-transformed wild-type strain.

引用

页码：105 / 110

页数：6

共 19 条

[1]

Burton K, 1968, METHODS ENZYMOLOGY B, V12, P163

[2]

CHATER KF, 1990, BIO-TECHNOL, V8, P115, DOI 10.1038/nbt0290-115

[3]

Corcoran JW, 1981, ANTIBIOTICS, P132

[4] MOLECULAR CHARACTERIZATION OF A GENE FROM SACCHAROPOLYSPORA-ERYTHRAEA (STREPTOMYCES-ERYTHRAEUS) WHICH IS INVOLVED IN ERYTHROMYCIN BIOSYNTHESIS [J].