FUNCTION OF SAPOSIN-C IN THE RECONSTITUTION OF GLUCOSYLCERAMIDASE BY PHOSPHATIDYLSERINE LIPOSOMES

被引：43

作者：

VACCARO, AM ^{[1
]}

TATTI, M ^{[1
]}

CIAFFONI, F ^{[1
]}

SALVIOLI, R ^{[1
]}

MARAS, B ^{[1
]}

BARCA, A ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,DEPT BIOCHEM SCI,I-00185 ROME,ITALY

来源：

FEBS LETTERS | 1993年 / 336卷 / 01期

关键词：

GLUCOSYLCERAMIDASE RECONSTITUTION; SAPOSIN C; PHOSPHATIDYLSERINE LIPOSOME;

D O I：

10.1016/0014-5793(93)81631-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of saposin C (Sap C), a glucosylceramidase activator protein, in the enzyme stimulation by phosphatidylserine (PS) liposomes has been investigated. Using gel filtration experiments evidence was obtained for Sap C binding to PS large unilamellar vesicles (LUV) but not to glucosylceramidase. PS LUV which by themselves are unable to tightly bind and stimulate the enzyme, acquire the capacity to also bind the enzyme after interaction with Sap C, making it express its full activity. Our results indicate that the primary step in the Sap C mode of action resides in its association with PS membranes; in turn, this association promotes the interaction between the membranes and glucosylceramidase.

引用

页码：159 / 162

页数：4

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