HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF CHLORAMBUCIL AND ITS N-OXIDE PRODRUG

被引：7

作者：

CHANDLER, KJ ^{[1
]}

MCCABE, JB ^{[1
]}

KIRKPATRICK, DL ^{[1
]}

机构：

[1] UNIV REGINA,DEPT CHEM,REGINA S4S 0A2,SASKATCHEWAN,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0389-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic method is described to distinguish chlorambucil N-oxide from the parent chlorambucil and quantitate both after separation from biological samples. The influence of solvent pH, alcohol, acid and ion-pairing agent on the separation is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discussed with potential application for metabolic studies.

引用

页码：195 / 202

页数：8

共 16 条

[1] ADAIR C G, 1986, Analytical Proceedings, V23, P30
[2] STUDIES ON THE MECHANISM OF GASTROINTESTINAL ABSORPTION OF MELPHALAN AND CHLORAMBUCIL
ADAIR, CG
MCELNAY, JC
[J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1986, 17 (01) : 95 - 98
[3] DETERMINATION OF CHLORAMBUCIL IN PLASMA USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
ADAIR, CG
BURNS, DT
CROCKARD, AD
HARRIOTT, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 342 (02): : 447 - 451
[4] DESIGN OF ALKYLATING AGENTS FOR SELECTIVITY OF ACTION
BERGEL, F
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1958, 68 (03) : 1238 - 1245
[5] BOSANQUET AG, 1986, CANCER CHEMOTH PHARM, V18, P176
[6] KINETICS OF CHLORAMBUCIL HYDROLYSIS USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
CHATTERJI, DC
YEAGER, RL
GALLELLI, JF
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1982, 71 (01) : 50 - 54
[7] DEGUTENE A, 1986, J ORG CHEM USSR, V22, P2191
[8] DEGRADATION OF CHLORAMBUCIL IN AQUEOUS-SOLUTION
EHRSSON, H
EKSBORG, S
WALLIN, I
NILSSON, SO
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1980, 69 (09) : 1091 - 1094
[9] GREIG NH, 1988, CANCER CHEMOTH PHARM, V21, P1
[10] PHYSICOCHEMICAL AND PHARMACOKINETIC PARAMETERS OF 7 LIPOPHILIC CHLORAMBUCIL ESTERS DESIGNED FOR BRAIN PENETRATION
GREIG, NH
GENKA, S
DALY, EM
SWEENEY, DJ
RAPOPORT, SI
[J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1990, 25 (05) : 311 - 319

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