COMPARISON OF 2 ACTIVE HET-A RETROPOSONS OF DROSOPHILA-MELANOGASTER

被引:8
作者
BIESSMANN, H [1 ]
KASRAVI, B [1 ]
BUI, T [1 ]
FUJIWARA, G [1 ]
CHAMPION, LE [1 ]
MASON, JM [1 ]
机构
[1] NIEHS, GENET GRP, RES TRIANGLE PK, NC 27709 USA
关键词
D O I
10.1007/BF00352317
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
HeT-A elements are Drosophila melanogaster LINE-like retroposons that transpose to broken chromosome ends by attaching themselves with an oligo(A) tail. Since this family of elements is believed to be involved in the vital function of telomere elongation in Drosophila, it is important to understand their transposition mechanism and the molecular aspects of activity. By comparison of several elements we have defined here the unit length of HeT-A elements to be approximately 6 kb. Also, we studied an active HeT-A element that had transposed very recently to the end of a terminally deleted X chromosome. The 12 kb of newly transposed DNA consisted of a tandem array of three different HeT-A elements joined by oligo(A) tails to each other and to the chromosome end broken in the yellow gene. Such an array may have transposed as a single unit or resulted from rapid successive transpositions of individual HeT-A elements. By sequence comparison with another recently transposed HeT-A element, conserved domains in the single open reading frame (ORF), encoding a gag-like polypeptide, of these elements were defined. We conclude that for transposition an intact ORF is required in cis, while the reverse transcriptase is not encoded on the HeT-A element but is provided in tl ans. This would make HeT-A elements dependent on an external reverse transcriptase for transposition and establish control of the genome over the activity of HeT-A elements. This distinguishes the Drosophila HeT-A element, which has been implicated in Drosophila telomere elongation, from the other, 'selfish' LINE-like elements.
引用
收藏
页码:90 / 98
页数:9
相关论文
共 49 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] BERG JM, 1990, J BIOL CHEM, V265, P6513
  • [3] THE GENOMIC ORGANIZATION OF HET-A RETROPOSONS IN DROSOPHILA-MELANOGASTER
    BIESSMANN, H
    KASRAVI, B
    JAKES, K
    BUI, T
    IKENAGA, K
    MASON, JM
    [J]. CHROMOSOMA, 1993, 102 (05) : 297 - 305
  • [4] GENETICS AND MOLECULAR-BIOLOGY OF TELOMERES
    BIESSMANN, H
    MASON, JM
    [J]. ADVANCES IN GENETICS, 1992, 30 : 185 - 249
  • [5] PROGRESSIVE LOSS OF DNA-SEQUENCES FROM TERMINAL CHROMOSOME DEFICIENCES IN DROSOPHILA-MELANOGASTER
    BIESSMANN, H
    MASON, JM
    [J]. EMBO JOURNAL, 1988, 7 (04) : 1081 - 1086
  • [6] FREQUENT TRANSPOSITIONS OF DROSOPHILA-MELANOGASTER HET-A TRANSPOSABLE ELEMENTS TO RECEDING CHROMOSOME ENDS
    BIESSMANN, H
    CHAMPION, LE
    OHAIR, M
    IKENAGA, K
    KASRAVI, B
    MASON, JM
    [J]. EMBO JOURNAL, 1992, 11 (12) : 4459 - 4469
  • [7] CHROMOSOME ENDS IN DROSOPHILA WITHOUT TELOMERIC DNA-SEQUENCES
    BIESSMANN, H
    CARTER, SB
    MASON, JM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) : 1758 - 1761
  • [8] ADDITION OF TELOMERE-ASSOCIATED HET DNA-SEQUENCES HEALS BROKEN CHROMOSOME ENDS IN DROSOPHILA
    BIESSMANN, H
    MASON, JM
    FERRY, K
    DHULST, M
    VALGEIRSDOTTIR, K
    TRAVERSE, KL
    PARDUE, ML
    [J]. CELL, 1990, 61 (04) : 663 - 673
  • [9] HET-A, A TRANSPOSABLE ELEMENT SPECIFICALLY INVOLVED IN HEALING BROKEN CHROMOSOME ENDS IN DROSOPHILA-MELANOGASTER
    BIESSMANN, H
    VALGEIRSDOTTIR, K
    LOFSKY, A
    CHIN, C
    GINTHER, B
    LEVIS, RW
    PARDUE, ML
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (09) : 3910 - 3918
  • [10] THE MOLECULAR-BASIS OF I-R HYBRID DYSGENESIS IN DROSOPHILA-MELANOGASTER - IDENTIFICATION, CLONING, AND PROPERTIES OF THE I-FACTOR
    BUCHETON, A
    PARO, R
    SANG, HM
    PELISSON, A
    FINNEGAN, DJ
    [J]. CELL, 1984, 38 (01) : 153 - 163