SINGLE-STRANDED AND DOUBLE-STRANDED RNAS AND PROTEINS OF INCOMPLETE INFLUENZA VIRUS

The development of von Magnus virus (vMv) with a strain of influenza virus was investigated from the standpoint of the characteristics of its RNA, both the single-stranded within the particle and the double-stranded from the infected cells. The proteins on the particles were also examined. The amount of radioactively labeled RNA that could be isolated from vMv particles of the second and third passages decreased over that of the first passage. The amount of labeled double-stranded RNA which could be isolated from the cells also declined sharply in subsequent vM passages while the decline of labeled coat protein was not so marked. The most significant qualitative change was the absence of the fifth peak of single-stranded RNA from the virus of the second von Magnus passage. © 1969.