A RAPID METHOD FOR PREPARING CRYSTALLINE HUMAN HEMOGLOBIN AND SEPARATION OF CRYSTALLINE HEMOGLOBIN A IN QUANTITY

被引：19

作者：

HINSON, JA

MCMEEKIN, TL

机构：

[1] Department of Biology University of South Carolina, Columbia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 35卷 / 01期

关键词：

D O I：

10.1016/0006-291X(69)90487-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method is described for the large scale preparation of crystalline human hemoglobin. The use of toluene and rapid stirring are essential for rapid crystallization. The minor hemoglobin component A2, present in crystalline hemoglobin, is removed by a batch procedure of adsorption on DEAE cellulose and fractionation by elution under conditions determined by column chromatography. © 1969.

引用

页码：94 / &

共 13 条

[1]

CANNAN RK, 1942, BLOOD SUBSTITUTES BL, P147

[2] A CHROMATOGRAPHIC STUDY OF THE MINOR COMPONENTS OF NORMAL ADULT HUMAN HEMOGLOBIN INCLUDING A COMPARISON OF HEMOGLOBIN FROM NORMAL AND PHENYLKETONURIC INDIVIDUALS [J].