CONTROL IN THE DARK OF THE PLASTOQUINONE REDOX STATE BY MITOCHONDRIAL ACTIVITY IN CHLAMYDOMONAS-REINHARDTII

被引:42
作者
GANS, P
REBEILLE, F
机构
[1] Service de Radioagronomie, Departement de Biologie, C.E.N. de Cadarache, Saint-Paul-lez-Durance
关键词
Fluorescence; Glycolysis; Mitochondrial respiration; Respiration and photosynthesis interaction; State I-State II transition;
D O I
10.1016/0005-2728(90)90226-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In Chlamydomonas reinhardtii, addition of mitochondrial oxidases inhibitors or uncouplers in the dark resulted in an increase of the variable fluorescence level, a decrease of the maximum fluorescence level and a rise of the slow electrochromic phase in flash-induced 520 nm absorption changes. Fluorescence emission spectra recorded at 77 K presented a marked decrease in the F686nm F715nm ratio which was suggestive of a state I-state II transition. Biochemical analysis indicated that the ATP and glucose 6-phosphate pools decreased markedly under these conditions, whereas the NADPH concentration increased. The NAD pool remained totally oxidised. These results strongly suggest that alterations of the mitochondrial oxidative phosphorylations in the dark could induce an increase of the reduction state of the plastoquinone pool and a redistribution of the antennae between the two photosystems. It is proposed that these variations were linked to an acceleration of the NADPH production in the chloroplast through the glycolytic activity. © 1990.
引用
收藏
页码:150 / 155
页数:6
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