DOUBLE LABELING IMMUNOHISTOCHEMICAL TECHNIQUE PROVIDES EVIDENCE OF THE SPECIFICITY OF GLIAL-CELL MARKERS

被引:122
作者
GHANDOUR, MS [1 ]
LANGLEY, OK [1 ]
VINCENDON, G [1 ]
GOMBOS, G [1 ]
机构
[1] CNRS,CTR PHYSIOL & MED BIOPHYS,F-67000 STRASBOURG,FRANCE
关键词
D O I
10.1177/27.12.118210
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Sequential treatment of rat cerebellar slices with two antisera, each specific for a different glial cell marker, revealed, by indirect immunofluorescence combined with the immunoperoxidase method on the same tissue section, that carbonic anhydrase isoenzyme II (CA II) is exclusively localized in oligodendrocytes while the glial fibrillary acidic protein (GFA) is, as well established, a specific marker for astrocytes.
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页码:1634 / 1637
页数:4
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