FUSION OF MITOCHONDRIA WITH PROTOPLASTS IN SACCHAROMYCES-CEREVISIAE

被引:27
作者
GUNGE, N [1 ]
SAKAGUCHI, K [1 ]
机构
[1] MITSUBISHI KASEI,INST LIFE SCI,TOKYO,JAPAN
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 170卷 / 03期
关键词
D O I
10.1007/BF00267057
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protoplasts prepared from a neutral petite haploid BO60AF-1 (a ade2 arg4 leu2 trp COEOOO υO ρO) were mixed with mitochondria isolated from an oligomycin resistant respiring haploid ANROR 12D (a his4 leu2 thr4 CSESOIIRυ+ ρ+) and treated with 30% polythylene glycol and CaCl2. When the treated protoplasts were spread and incubated on selective agar plates, oligomycin resistant respiration-sufficient colonies appeared with low frequency. All of these colonies carried the mitochondrial genotype of CSESOIIRυ+ ρ+ and showed the same mating type and nutritional requirements as did BO60AF-1, thus evidencing the mitochondrial transfer into protoplasts. Recombination and transmission of the mitochondrial drug resistance markers were studied in crosses involving the strains issued from mitochondria accepted protoplasts. © 1979 Springer-Verlag.
引用
收藏
页码:243 / 247
页数:5
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